Acta Virologica Vol.60, No.4, p.347-353, 2016

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.60, No.4, p.347-353, 2016
Title: Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y
Author: N. SIHELSKÁ, L. PREDAJŇA, A. NAGYOVÁ, K. ŠOLTYS, J. BUDIŠ, J. GUBIŠ, M. MRKVOVÁ, J. KRAIC, D. MIHÁLIK, M. GLASA

Abstract: Total RNAs from a symptomless tomato plant were subjected to next-generation sequencing (NGS) analysis, revealing the presence of a single viral agent – potato virus Y (PVY). The analysis of determined full-length genome sequence assigned the PVY SL16 isolate to the recombinant PVY-N-Wi strain group. A series of primers targeting the four main recombinant junction (RJ) sites were used for characterization of additional 5 tomato PVY isolates recovered in Western Slovakia. Based on the partial sequences, the isolates could be classified as belonging to PVY-N-Wi and PVY-NTNa strain groups. Interestingly, both these distinct recombinant PVY types were identified in mixed infection in one tomato sample (SL31). Our results further reinforce the data on the complexity of PVY infection and confirm the recombination as a significant evolutionary process shaping the PVY diversity.

Keywords: potyvirus; Solanum lycopersicum; detection; recombination; NGS
	Published online: 07-Dec-2016
Year: 2016, Volume: 60, Issue: 4	Page From: 347, Page To: 353
doi:10.4149/av_2016_04_347
	download file

Aepress.sk