Home Acta Virologica 2016 Acta Virologica Vol.60, No.4, p.347-353, 2016

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.60, No.4, p.347-353, 2016

Title: Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y
Author: N. SIHELSKÁ, L. PREDAJŇA, A. NAGYOVÁ, K. ŠOLTYS, J. BUDIŠ, J. GUBIŠ, M. MRKVOVÁ, J. KRAIC, D. MIHÁLIK, M. GLASA

Abstract: Total RNAs from a symptomless tomato plant were subjected to next-generation sequencing (NGS) analysis, revealing the presence of a single viral agent – potato virus Y (PVY). The analysis of determined full-length genome sequence assigned the PVY SL16 isolate to the recombinant PVY-N-Wi strain group. A series of primers targeting the four main recombinant junction (RJ) sites were used for characterization of additional 5 tomato PVY isolates recovered in Western Slovakia. Based on the partial sequences, the isolates could be classified as belonging to PVY-N-Wi and PVY-NTNa strain groups. Interestingly, both these distinct recombinant PVY types were identified in mixed infection in one tomato sample (SL31). Our results further reinforce the data on the complexity of PVY infection and confirm the recombination as a significant evolutionary process shaping the PVY diversity.

Keywords: potyvirus; Solanum lycopersicum; detection; recombination; NGS
Published online: 07-Dec-2016
Year: 2016, Volume: 60, Issue: 4 Page From: 347, Page To: 353
doi:10.4149/av_2016_04_347


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