Home 
Acta Virologica 2016 Acta Virologica Vol.60, No.4, p.410-416, 2016
Acta Virologica 2016 Acta Virologica Vol.60, No.4, p.410-416, 2016
Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Current articles 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudba 2025Webshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Acta Virologica Vol.60, No.4, p.410-416, 2016 |
||
| Title: Function of myosin during entry and egress of equid herpesvirus type 1 in primary murine neurons | ||
| Author: J. CYMERYS, A. SŁOŃSKA, J. SKWARSKA, M. W. BAŃBURA | ||
| Abstract: Equid herpesvirus type 1 (EHV-1) is a major pathogen of horses with a worldwide distribution, which can cause various clinical signs ranging from mild respiratory disease to neurological disorders. To initiate an effective infection, EHV-1 evolved a broad spectrum of mechanisms exploiting the host cell, including its actin filaments. An actin–myosin-driven transport has been described to precede cellular entry of different viruses. Therefore, in the present study we investigated the role of actin motor protein - myosin, during replication of two EHV-1 strains: Jan-E (wild-type EHV-1 strain isolated from aborted equine fetus) and Rac-H (attenuated strain highly adapted in cell cultures in vitro) in primary murine neurons. In order to investigate this, we used two inhibitors: blebbistatin (BLB; non-muscle myosin II inhibitor) and 2,3-butanedione monoxime (BDM; inhibitor of myosin ATPase). Our results demonstrated that limitation of Jan-E EHV-1 replication occurred in cells treated with myosin inhibitor, which confirmed the important role of actin motor proteins during the entry and egress of EHV-1 virions. Application of blebbistatin did not affect Rac-H EHV-1 replication, while BDM caused reduction of replication in murine neurons. Based on these results it can be assumed that EHV-1 virion movement was myosin-dependent. |
||
| Keywords: equid herpesvirus 1 (EHV-1); primary murine neurons; myosin; blebbistatin (BLB); 2,3-butanedione monoxime (BDM) | ||
| Published online: 07-Dec-2016 | ||
| Year: 2016, Volume: 60, Issue: 4 | Page From: 410, Page To: 416 | |
| doi:10.4149/av_2016_04_410 |
||
|
|
 download file |
|