Acta Virologica Vol.60, No.4, p.426-428, 2016

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Acta Virologica Vol.60, No.4, p.426-428, 2016
Title: Molecular detection of murine gammaherpesvirus 68 (MHV-68) in Haemaphysalis concinna ticks collected in Slovakia
Author: M. VRBOVÁ, P. BELVONČÍKOVÁ, A. KOVAĽOVÁ, R. MATÚŠKOVÁ, M. SLOVÁK, M. KÚDELOVÁ

Abstract: Murine gammaherpesvirus 68 (MHV-68) is a natural pathogen of murid rodents, which serve as hosts to Haemaphysalis concinna ticks. The occurrence of MHV-68 was investigated in a total of 47 H. concinna adult ticks collected on the vegetation in Gabčíkovo, situated in south-western Slovakia (47º54´0´´N, 17º35´0´´E), from May 2013 to May 2014. DNA from ticks was purified and screened by nested PCR targeting ORF50 of MHV-68 and the copy number of virus genome in ticks was determined by a real-time PCR assay specific for ORF65. The MHV-68 incidence in questing ticks was 38.3% (18/47) and the virus genome copy number per tick varied from 2x102 to 9.6x103. In this study, MHV-68 was documented for the first time in H. concinna ticks. Results expand previous data describing the occurrence of MHV-68 in Ixodes ricinus and Dermacentor reticulatus ticks collected in Slovakia, supporting the hypothesis that MHV-68 might be a newfound pathogen in ticks.

Keywords: murine herpesvirus 68; Haemaphysalis concinna ticks; nested PCR; Slovakia
	Published online: 07-Dec-2016
Year: 2016, Volume: 60, Issue: 4	Page From: 426, Page To: 428
doi:10.4149/av_2016_04_426
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