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Endocrine Regulations 2004 Endocrine Regulations Vol.38, p.1-6, 2004
Endocrine Regulations 2004 Endocrine Regulations Vol.38, p.1-6, 2004
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Endocrine Regulations Vol.38, p.1-6, 2004 |
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| Title: PITUITARY SYNAPTIC PROTEIN SNAP-25 SENSITIVE TO GnRH IS NECESSARY FOR LH RELEASE | ||
| Author: J. Luis Quintanar, Eva Salinas, Rosa María Chávez-Morales, Andrés Quintanar-Stephano | ||
| Abstract: Objective. The protein SNAP-25 is located in the plasma membrane and is known to participate in hormone exocytosis process. In the present work we studied the role of SNAP-25 on LH secretion in permeabilized adenohypophyseal cultured cells. The question of whether GnRH regulates SNAP-25 expression in adenohypophyseal cultured cells and in the adenohypophyses in vivo was also investigated.
Methods. In digitonin-permeabilized cells incubated with anti-SNAP-25, stimulated LH secretion with Ca2+ was analysed. The presence and expression of SNAP-25 in adenohypophyseal cultured cells incubated with GnRH and in adenohypophyses of orchidectomized rats with GnRH administration was studied by immunochemistry and immunoblotting.
Results. Immunochemical study revealed that SNAP-25 was present in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats both with GnRH treatment. We found that LH secretion can be blocked by antobodies raised against SNAP-25 in permeabilized cells. Likewise, GnRH administration induced a significant decrease of SNAP-25 expression in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats.
Conclusion. Our study showed that SNAP-25 is present in adenohypophyses in vitro as well as in vivo and that is involved in LH release and that GnRH can modify its expression. |
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| Keywords: Gonadotrophs – Immunohistochemistry – Immunoblot – Permeabilized cells – Digitonin | ||
| Year: 2004, Volume: 38, Issue: | Page From: 1, Page To: 6 | |
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