Acta Virologica Vol.61, No.1, p.97-104, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.1, p.97-104, 2017
Title: In vitro evaluation of a polytope DNA construct as a novel DNA vaccine strategy against human cytomegalovirus-associated diseases
Author: E. SABBAGHIAN, F. ROODBARI, J. AMANI, A. RAFIEI

Abstract: Human cytomegalovirus (HCMV) establishes severe disease in fetus, newborn and immunocompromised individuals. Polytope DNA vaccine strategy allows us to choose conserved and immunodominant epitopes from different antigens that can stimulate cellular and humoral immune responses simultaneously. In this study, a synthetic chimeric gene fragment was subcloned in to DNA vaccine vector pcDNA3.1+. The recombinant vector was transferred in to suitable eukaryotic cell line HEK 293T and the expression level of polytope construct from HEK 293T-infected cells was determined by western blot. These results show that there was no mutantion in target segment and recombinant vector showed significant levels of expression. Base on these results, using a proper procedure for design can cause expression and stability of polytope peptide.

Keywords: human cytomegalovirus; polytope DNA vaccine; immunodominant epitopes; sequencing; polytope construct; overexpression
	Published online: 28-Feb-2017
Year: 2017, Volume: 61, Issue: 1	Page From: 97, Page To: 104
doi:10.4149/av_2017_01_97
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