Home CONTACT Acta Virologica 2017 Acta Virologica Vol.61, No.1, p.105-115, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.61, No.1, p.105-115, 2017

Title: PVX-tolerant potato development using a nucleic acid-hydrolyzing recombinant antibody
Author: J.-G. YANG, K.-H. HWANG, E.-J. KIL, J. PARK, S. CHO, Y.-G. LEE, C.-K. AUH, Y. RHEE, S. LEE

Abstract: 3D8 scFv, a catalytic recombinant antibody developed in the MRL mouse, exhibits nucleic acid-hydrolyzing activity. Previous studies have demonstrated that tobacco plants harboring 3D8 scFv antibodies showed broad-spectrum resistance to infection by both DNA and RNA viruses. In this study, potatoes were transformed with the 3D8 scFv gene and screened by potato virus X (PVX) challenge. Starting with the T0 and T1 potato lines, PVX-tolerant T1 potatoes were identified in the field and characterized by ELISA and RT-PCR analysis. T2 potatoes were propagated for T3 generation and additional virus challenges in the field, and 44% of the 3D8 scFv T3 transgenic potatoes grown in GMO fields were found to be tolerant to PVX infection. Tubers from PVX-tolerant T3 lines were 60% bigger and 24% heavier, compared with tubers from PVX-susceptible transgenic lines and wild-type potatoes. Three-step virus challenge experiments and molecular characterization techniques were used for plants grown in growth chambers or fields to identify 3D8 scFv-transgenic, PVX-tolerant potatoes. These studies also revealed that the viral tolerance enabled by 3D8 scFv persisted during asexual propagation.

Keywords: potato virus X; 3D8 scFv; agrobacterium-mediated transformation; transgenic potato; PVX tolerance
Published online: 28-Feb-2017
Year: 2017, Volume: 61, Issue: 1 Page From: 105, Page To: 115

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