Home CONTACT Acta Virologica 2017 Acta Virologica Vol.61, No.1, p.123-126, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.1, p.123-126, 2017

Title: Recombinant expression of Mimivirus L725 ORFan gene product
Author: H. SOBHY, G. GOTTHARD, E. CHABRIÈRE, D. RAOULT, P. COLSON

Abstract: Mimivirus was the first discovered amoebal giant virus. The Mimivirus virions are covered by a dense layer of approximately 130 nm-long fibers, the length and shape of which diverge from those of other viruses. Here, we aimed at expressing the L725 protein to further confirm and study its role as a fiber-associated protein. We report Escherichia coli expression of the L725 protein, which is encoded by a Mimivirus ORFan, was previously identified by proteomics in purified viral fibers and demonstrated to be a fiber-associated protein by RNA-silencing experiments. The expressed protein was recognized by anti-Mimivirus fiber or anti-Mimivirus L725 polyclonal antibodies. This study is the only expression, to our knowledge, of a product from a Mimiviral ORFan gene.

Keywords: Mimivirus; giant virus; fiber; ORFan; gene expression
Published online: 28-Feb-2017
Year: 2017, Volume: 61, Issue: 1 Page From: 123, Page To: 126
doi:10.4149/av_2017_01_123


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