Home General Physiology and Biophysics 2017 General Physiology and Biophysics Vol.36, No.2, p.211–218, 2017

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.36, No.2, p.211–218, 2017

Title: Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells
Author: Meric A. Esmekaya, Ayşe G. Canseven, Handan Kayhan, Mehmet Z. Tuysuz, Bahriye Sirav, Nesrin Seyhan

Abstract: This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (ΔΨm) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determined by the MTT test. Cytochrome-c and p53 levels were determined by an ELISA method. The relative ΔΨm was analysed by JC-1 staining using flow cytometer. Apoptotic rate of the cells was measured by Annexin-V-FITC staining. All assays were performed after certain time of incubations (15 min–4 h) following MW exposure. MW-exposed cells showed a significant decrease in viability when compared to unexposed cells. A significantly larger decrease was observed after longer exposure. The percentage of apoptotic cells, amount of cytochrome-c, and relative ΔΨm were significantly higher in MW-exposed cells. The percent of apoptotic cells and relative ΔΨm in 24 h MW-exposed group was significantly higher than those in 4 h MW-exposed group. However, no significant change was observed in p53 levels. These results demonstrated that exposure to 2.1-GHz WCDMA-modulated MW radiation caused hyperpolarization of mitochondria that in turn induced apoptosis in MCF-7 cells.

Keywords: Microwave radiation — Mitochondrial membrane potential — Apoptosis — Cytochrome-c — p53
Published online: 07-Mar-2017
Year: 2017, Volume: 36, Issue: 2 Page From: 211, Page To: 218

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