Home HOME Endocrine Regulations 2004 Endocrine Regulations Vol.38, p.51-55, 2004

Journal info

Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

Aims and Scope
Editorial Info
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Endocrine Regulations Vol.38, p.51-55, 2004

Title: IN VITRO EFFECTS OF INHIBIN ON APOPTOSIS AND APOPTOSIS RELATED PROTEINS IN HUMAN OVARIAN GRANULOSA CELLS
Author: R. DENKOVA, V. BOURNEVA, L. STANEVA-DOBROVSKI, E. ZVETKOVA, K. BALEVA, E. YANEVA, B. NIKOLOV, I. IVANOV, K. SIMEONOV, T. TIMEVA, M. YANKOV

Abstract: Objective. To clarify the in vitro effects of inhibin A (I) on apoptotic cell death and its mechanisms in ovarian granulosa cells the immunoexpression patterns of the apoptosis markers caspase- 3 and pro- and anti-apoptotic proteins (Bcl-2, Bcl-xl, Bak) were evaluated in ovarian granulosa cells collected from women with different hormonal status. Materials and Methods. Granulosa cells were isolated from follicles of women participating in an in vitro fertilization (IVF) program, normally cyclic (NC) and premenopausal women (PrM). The obtained cells were cultured for 72 h with inhibin A (Sigma, USA) – 10 ng/ml. The concentration of estradiol in the culture medium was determined by radioimmunoassay using the Coat-ACount kit (Nippon, Japan), whose intra- and interassay coefficients of variations were 6,8 % and 6,2 % respectively. The expression of caspase-3, Bak, Bcl-2, Bcl-xl was investigated immunohistochemically. The percentages of immunopositive cells were calculated and Student’s t-test was used for statistical analysis. Results. Addition of inhibin A (10 ng/ml) to granulosa cells cultures resulted in increased estradiol production. Maximal stimulation was observed in granulosa cells collected from women participating in IVF whereas minimal effect of inhibin treatment on estradiol production was demonstrated in premenopausal women. Inhibin A exposition enhanced the immunoexpression of prooncogenes (Bcl-2, Bcl-xl) and reduced the expression of caspase-3 and pro-apoptotic protein Bak in ovarian granulosa cells from the three experimental groups. Conclusions. Our findings suggest that inhibin A in vitro stimulates the estradiol secretion by granulosa cells dependently of the woman hormonal status, while it inhibits apoptotic process in granulosa cells independently of the hormonal status.

Keywords: Human ovarian granulosa cells – Inhibin – Apoptosis – Apoptosis-related proteins H
Year: 2004, Volume: 38, Issue: Page From: 51, Page To: 55



download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.