Home Acta Virologica 2017 Acta Virologica Vol.61, No.2, p.175-182, 2017

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.61, No.2, p.175-182, 2017

Title: Genetic characterization of canine parvovirus from dogs in Pakistan
Author: M. Z. SHABBIR, M. U. SOHAIL, U. N. CHAUDHARY, W. YAQUB, I. RASHID, M. H. SALEEM, M. MUNIR

Abstract: Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2–6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64.71%) were found positive both in hemagglutination and PCR assays. Phylogenetic and evolutionary analysis demonstrated higher genetic heterogeneity in studied strains and constituted seven clusters within the CPV-2a group, however, they shared high level of identity with Chinese strains. Further studies are necessary to elucidate genetic analysis and epidemiology of CPV variants across a wide geographical area of the country.

Keywords: canine parvovirus; dog; CPV-2a; Pakistan
Published online: 19-May-2017
Year: 2017, Volume: 61, Issue: 2 Page From: 175, Page To: 182
doi:10.4149/av_2017_02_06


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