Home Neoplasma 2017 Neoplasma Vol.64, No.4, p.554-562, 2017

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

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Neoplasma Vol.64, No.4, p.554-562, 2017

Title: MicroRNA-300 targets hypoxia inducible factor-3 alpha to inhibit tumorigenesis of human non-small cell lung cancer
Author: Y. Zhang, Y. Guo, C. Yang, S. Zhang, X. Zhu, L. Cao, W. Nie, H. Yu

Abstract: Non-small cell lung cancer (NSCLC) is one of the most deadly human cancers. MicroRNA-300 acts as both tumor promoter and suppressor in different types of cancer. Here, we try to identify the function of microRNA-300 in human NSCLC. We compared MicroRNA-300 levels between tumor tissues versus paired adjacent non-tumor lung tissues from NSCLC patients, and in NSCLC versus normal lung cell lines. Effects of microRNA-300 on cell proliferation, invasion and migration were examined in vitro, and on tumor growth in vivo using a xenograft mouse model. Potential mRNA targets of microRNA-300 were predicted and underlying mechanism was explored. MicroRNA-300 expression was lower in both NSCLC tissues and cell lines. Overexpression of microRNA-300 inhibited proliferation, invasion and migration of NSCLC cells in vitro, and tumor growth in vivo. MicroRNA-300 could directly bind to the 3’-UTR of hypoxia inducible factor-3 alpha (HIF3α) mRNA, and inhibit both its mRNA and protein expressions. Restoring HIF3α expression could rescue the inhibitory effects of microRNA-300 on tumorigenesis of NSCLC both in vitro and in vivo. MicroRNA-300 is a tumor suppressor microRNA in NSCLC by downregulating HIF3α expression. Both microRNA-300 and HIF3α may serve as potential therapeutic targets in NSCLC treatment.

Keywords: Non-small cell lung cancer, microRNA-300, tumor, xenograft, hypoxia inducible factor-3 alpha
Published online: 11-Jul-2017
Year: 2017, Volume: 64, Issue: 4 Page From: 554, Page To: 562

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