Home Bratislava Medical Journal 2017 Bratislava Medical Journal Vol.118, No.5, p.283-287, 2017

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.118, No.5, p.283-287, 2017

Title: Which one is more effective for the treatment of rat sepsis model: thalidomide or etanercept?
Author: N. Ilhan, S. Susam, H. F. Gul, R. Bardas, N. Ilhan


OBJECTIVE: We aimed to investigate the protective effect of selected treatment agents on liver injury in lipopolysaccharide (LPS)-induced rat sepsis model.
BACKGROUND: The sepsis includes complex inflammatory responses between a microbial pathogen and the host immune system, and leads to organ failure and also death.
METHODS: This study was performed with 29 male Wistar Albino rats. Rats were divided randomly into five groups: Sham group, LPS-treated sepsis group, LPS+thalidomide treated group, LPS+etanercept treated group and LPS+thalidomide+etanercept treated group, respectively. Liver tissue tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) levels were determined by enzyme-linked immuno-sorbent assay (ELISA) method. The expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) was performed using western blot analysis.
RESULTS: The levels of tissue TNF-α, IL-1β and IL-6 were found statistically significantly higher in sepsis group than in the sham group. TNF-α levels were found statistically significantly decreased in LPS+etanercept and LPS+thalidomide+etanercept treated groups when compared with LPS group (p < 0.05). For IL-1β and IL-6 levels a statistically significant decline was observed in the LPS+thalidomide and LPS+etanercept treated groups compared to the LPS group (p < 0.05).

Expression of NF-κB protein in liver tissue was significantly elevated in the LPS group compared to sham group (p < 0.001). In treatment groups, a marked decrease was observed in NF-κB protein expression.
CONLUSION: The results of this investigation suggested that etanercept and thalidomide administration may have a beneficial effect on LPS-induced sepsis. So, the present study may have significant clinical relevance, but clinical trials are needed to confirm these results (Tab. 1, Fig. 1, Ref. 36).

Keywords: sepsis, IL-1β, IL-6, TNF-α, treatment agents
Published online: 15-May-2017
Year: 2017, Volume: 118, Issue: 5 Page From: 283, Page To: 287

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