Home Endocrine Regulations 2004 Endocrine Regulations Vol.38, p.131-142, 2004

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Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

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Endocrine Regulations Vol.38, p.131-142, 2004

Author: Chang Li, Yoshifumi Hirooka, Satoshi Habu, Junko Takagi, Minehiro Gotoh, Tsuyoshi Nogimori

Abstract: Objective. To identify the distribution of thyrostimulin, a heterodimer of glycoprotein hormone subunits (A2 and B5) by immunohistochemistry in the rat tissues using specific antipeptide antiserum which we recently produced. Method. Anti-thyrostimulin antibody was raised in New Zealand white rabbits immunized with a conjugate of synthetic A2 or B5 with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method. Results. Thyrostimulin immunoreactivity was visualized in the anterior pituitary, central nervous system, adrenal gland, stomach, duodenum, pancreas and testis. When using antiserum preincubated with synthetic peptides or rat pituitary homogenate which contains thyrostimulin peptide, no significant stain of the pituitary was detected. Conclusion. These findings suggest that thyrostimulin is widely distributed and that the method used is valuable in studying the distribution of thyrostimulin in rats.

Keywords: Thyrostimulin – Immunohistochemistry – Glycoprotein hormone subunits – A2 antibody – B5 antibody
Year: 2004, Volume: 38, Issue: Page From: 131, Page To: 142

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