Endocrine Regulations Vol.38, p.161-166, 2004

Quarterly, 50 pp. per issue
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

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Endocrine Regulations Vol.38, p.161-166, 2004
Title: EFFECT OF LEPTIN ON PROLIFERATIVE ACTIVITY AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) SECRETION FROM CULTURED ENDOTHELIAL CELLS HECA10 IN VITRO
Author: Beata Misztal-Dethloff, Henryk Stepien, Jan Komorowski

Abstract: Objective. The aim of this study was to check if leptin influences the proliferative activity and vascular endothelial grofth factor (VEGF) release from cultured mouse endothelial cells in vitro. Methods. The murine cell line HECa10 obtained from endothelial cells of mouse peripheral lymph nodes immortalised by transfection of plasmid with the gene for the large T antigen of Simian virus 40 was used in the experiments. The proliferative activity of HECa10 cells was studied by Mosmann and VEGF release by ELISA methods. Results. Murine leptin in concentrations from 5 to 25 ng/ml stimulated the proliferative activity of 72 h endothelial cell cultures and in concentrations from 0.5 to 25 ng/ml augmented the release of VEGF into supernatants of 24 h and 72 h cultured cells. Conclusion. Leptin stimulated proliferation and VEGF secretion of endothelial cells in vitro.

Keywords: Angiogenesis – Leptin – Endothelial cells – Vascular endothelial growth factor

Year: 2004, Volume: 38, Issue:	Page From: 161, Page To: 166

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