Home HOME Acta Virologica 2017 Acta Virologica Vol.61, No.3, p.252-263, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.3, p.252-263, 2017

Title: Detection and molecular characterization of tomato yellow leaf curl virus naturally infecting Lycopersicon esculentum in Egypt
Author: M. RABIE, C. RATTI, E. ABDEL ALEEM, F. FATTOUH

Abstract: Tomato yellow leaf curl virus (TYLCV) infections of tomato crops in Egypt were widely spread in 2014. Infected symptomatic tomato plants from different governorates were sampled. TYLCV strains Israel and Mild (TYLCV-IL, TYLCV-Mild) were identified by multiplex and real-time PCR. In addition, nucleotide sequence analysis of the V1 and V2 protein genes, revealed ten TYLCV Egyptian isolates (TYLCV from TY1 to 10). Phylogenetic analysis showed their high degree of relatedness with TYLCV-IL Jordan isolate (98%). Here we have showed the complete nucleotide sequence of the TYLCV Egyptian isolate TY10, sampled from El Beheira. A high degree of similarity to other previously reported Egyptian isolates and isolates from Jordan and Japan reflect the importance of phylogenetic analysis in monitoring virus genetic diversity and possibilities for divergence of more virulent strains or genotypes.

Keywords: multiplex PCR; real-time PCR; TYLCV-IL; TYLCV-Mild; phylogenetic analysis
Published online: 28-Aug-2017
Year: 2017, Volume: 61, Issue: 3 Page From: 252, Page To: 263
doi:10.4149/av_2017_303


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