Home HOME Acta Virologica 2017 Acta Virologica Vol.61, No.3, p.308-315, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.3, p.308-315, 2017

Title: Herpes simplex viruses type 1 and 2 photoinactivated in the presence of methylene blue transform human and mouse cells in vitro
Author: M. MICHÚTOVÁ, V. MRÁZOVÁ, M. KÚDELOVÁ, M. SMOLINSKÁ, M. ŠUPOLIKOVÁ, M. VRBOVÁ, F. GOLAIS

Abstract: Three strains of herpes simplex virus, K17syn- and HSZPsyn+ of type 1 (HSV-1) and USsyn- of type 2 (HSV-2), were photoinactivated in the presence of methylene blue and used to infect 3 cell lines, normal human lung tissue cells (MRC-5), mouse epithelial cells (NIH3T3), and human lung carcinoma cells (A549). The virus titer and phenotype of cells were evaluated to compare the characteristics of normal and carcinoma cells infected with non-syncytial (non-syn) and syncytial (syn) strains of herpes simplex viruses. We found that the cells of both normal cell lines infected with photoinactivated K17syn- and USsyn- but not HSZPsyn+ acquired transformed phenotype accompanied by the presence of virus. Surprisingly, the infection with photoinactivated viruses K17syn- and USsyn- but not HSZPsyn+ resulted in the suppression of the transformed phenotype of A549 cells. Using nested PCR, herpesviral DNA was identified in newly transformed cells and cells that lost the transformed phenotype. The effect of putative herpesvirus-related growth factors (HRGF) produced by cells infected with photoinactivated viruses was quantified and compared. Since methylene blue is currently used in phototherapy of herpetic lesions, these results raise the question of whether such therapy is risky to human health.

Keywords: herpes simplex viruses; photoinactivation of viruses; methylene blue; putative herpesvirus-related growth factors; transformed phenotype; phototherapy
Published online: 28-Aug-2017
Year: 2017, Volume: 61, Issue: 3 Page From: 308, Page To: 315
doi:10.4149/av_2017_309


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