Home HOME Acta Virologica 2017 Acta Virologica Vol.61, No.3, p.349-352, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.3, p.349-352, 2017

Title: RGB color coded images in scanning electron microscopy of biological surfaces
Author: O. KOFROŇOVÁ, O. BENADA

Abstract: We present here a methodological approach for the creation of color images in scanning electron microscopy by processing grayscale images taken simultaneously from at least three different detectors in a scanning electron microscope. The final color images are then produced by merging together those grayscale images in RGB color space. We show the images from non-conductive standard sample together with those obtained from real microbiological samples. The first one represents a microbial biofilm naturally grown on fiber glass filter. The other shows individual Bacillus subtilis cells from batch culture. All the image handling was done in open source image processing software ImageJ or GNU Image Manipulation Program (Gimp) or, alternatively, in proprietary AnalySis 3.2 Pro software processing suite.

Keywords: biological surfaces; color images; high resolution; scanning electron microscopy
Published online: 28-Aug-2017
Year: 2017, Volume: 61, Issue: 3 Page From: 349, Page To: 352
doi:10.4149/av_2017_315


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