Home FOR AUTHORS Acta Virologica 2017 Acta Virologica Vol.61, No.3, p.361-368, 2017

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.3, p.361-368, 2017

Title: Protein composition of the phase I Coxiella burnetii soluble antigen prepared by extraction with trichloroacetic acid
Author: G. FLORES-RAMÍREZ, M. KMEŤOVÁ, M. DANCHENKO, E. ŠPITALSKÁ, V. HAVLÍČEK, Ľ. ŠKULTÉTY

Abstract: Q fever is a highly infectious, widespread airborne zoonosis caused by Coxiella burnetii bacterium. Humans usually acquire the disease by inhalation of contaminated aerosol produced by infected livestock. Vaccination is the most practical way for prevention and control of the disease in the exposed population. In this work, we reviewed the most important Q-fever outbreaks in Slovakia as well as the progress in vaccine development. One of them represents a soluble antigen complex produced by extraction with trichloroacetic acid from a highly purified C. burnetii phase I strain Nine Mile. It was developed at the Institute of Virology in Bratislava. The protein content of this vaccine was separated by gel electrophoresis and analyzed by mass spectrometry. The study has resulted in the identification of 39 bacterial proteins from which 12 were recognized as immunoreactive. Most of the proteins were involved in bacterium pathogenicity (41.6%) and cell wall maintenance (25%). Four of the immunoreactive proteins may possess the moonlighting activity. Definition of the vaccine components represents a prerequisite for vaccine standardization and approval by governmental authorities.

Keywords: Coxiella burnetii; Q fever; outbreaks in Slovakia; soluble antigen; chemovaccine; trichloroacetic acid; protein composition
Published online: 28-Aug-2017
Year: 2017, Volume: 61, Issue: 3 Page From: 361, Page To: 368
doi:10.4149/av_2017_317


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