Home FOR AUTHORS Acta Virologica 2017 Acta Virologica Vol.61, No.4, p.473-478, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.4, p.473-478, 2017

Title: Yeast two-hybrid and pull-down assays propose an interaction between P50 of apple chlorotic leaf spot virus and PR-10 of Malus sylvestris cv. R12740-7A
Author: Y. Wang, N. Li, X. Zhao, J. Hu, Y. He, T. Hu, S. Wang, Y. Wang, K. Cao

Abstract: Apple chlorotic leaf spot virus (ACLSV) movement protein (P50) is involved in cell-to-cell transport and influences the long-distance spread of silencing activity. Previously, we obtained 69 P50-interacting proteins from Malus sylvestris cv. R12740-7A and using bioinformatics analyzed their biological functions. In this study, we used the GAL4-based two-hybrid yeast system and His pull-down assays to confirm an interaction between PR-10 of M. sylvestris cv. R12740-7A and ACLSV P50. Our results provide a theoretical basis for further research on the biological function of PR-10 in ACLSV infection and the interacting mechanism between host and virus.

Keywords: apple chlorotic leaf spot virus; Malus sylvestris cv. R12740-7A; P50; PR-10
Published online: 17-Nov-2017
Year: 2017, Volume: 61, Issue: 4 Page From: 473, Page To: 478
doi:10.4149/av_2017_406


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