Home CONTACT Acta Virologica 2017 Acta Virologica Vol.61, No.4, p.483-486, 2017

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.61, No.4, p.483-486, 2017

Title: First report of bovine herpesvirus 1 isolation from bull semen samples in China
Author: L. ZHU, Y. YU, X. JIANG, W. YUAN, G. ZHU

Abstract: Bovine herpesvirus 1 (BoHV-1) infection causes substantial economic losses to the cattle industry worldwide. So far, the isolation of BoHV-1 field virus has not been reported in China. Here, for the first time we report that two isolates of BoHV-1 designated as NJ16-1 and NJ16-2 were obtained from semen samples from breeding bulls in China. Typical cytopathic effect in MDBK cells, detection of viral protein VP16 in western blot analysis, PCR detection of BoHV-1 gB gene proved BoHV-1 infection and subsequent nucleotide sequence analysis showed a 99% identity with BoHV-1 Cooper strain. These results suggest that these isolated viruses are BoHV-1.

Keywords: BoHV-1; virus isolation; VP16; CPE
Published online: 17-Nov-2017
Year: 2017, Volume: 61, Issue: 4 Page From: 483, Page To: 486
doi:10.4149/av_2017_411


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