Home Bratislava Medical Journal 2017 Bratislava Medical Journal Vol.118, No.10, p.609-612, 2017

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

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Bratislava Medical Journal Vol.118, No.10, p.609-612, 2017

Title: Proprotein convertase 1 mediated proneuropeptide proteolytic processing in ischemic neuron injury
Author: S. S. Tang, Z. Y. Liang, L. R. Guo, J. H. Zhang, D. Zhou


BACKGROUND: Pro-protein processing mechanism plays an important role in neuron injury.
OBJECTIVE: To study the protein convertase 1 (PC1) mediated processing mechanism, the ischemic cellular or tissue proPC1/PC1 or proCgA/CgA (pro-chromogranin A) was analyzed.
METHODS: NS20Y differentiated cells were stressed by 0–6 h of oxygen and glucose deprivation (OGD) in glucose-free DMEM and an anaerobic jar environment. Ischemic C57BL/J mouse model was established by performing 60-min of middle cerebral artery occlusion (MCAO) operation and subsequent 4 or 24-h reperfusion. The TUNEL, immunochemistry, and Western blot methods were used to detect protein expression in ischemic cells or tissues.
RESULTS: The OGD or MCAO stress caused substantial cell death in a dose-dependent manner (p < 0.05 or 0.01).

With the increasing OGD dose, proPC1 and PC1 proteins gradually increased (p < 0.05 or 0.01) whereas proCgA and CgA proteins decreased (p < 0.05). In vivo the proPC1 and PC1 expressions presented with a peak at 4-h and then decreased at 24-h reperfusion (p < 0.05 or 0.01). The tissue proCgA and CgA proteins decreased with the increasing reperfusion time (p < 0.05).
CONCLUSIONS: The results suggest that the increasing PC1 expression promoted the transformation of proCgA into CgA or smaller peptides, i.e. Pancreastatin or Secretoneurin, and the PC1 mediated processing plays a critical role (Fig. 4, Ref. 15).

Keywords: pro-protein processing, neuron ischemic injury, pro-protein convertase 1, chromogranin A
Published online: 13-Nov-2017
Year: 2017, Volume: 118, Issue: 10 Page From: 609, Page To: 612

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