Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.118, No.11, p.702-704, 2017 |
||
Title: Optimization of Eva Green real-time mPCR for differentiating C. jejuni/coli directly from feces | ||
Author: M. Pavlova, V. Velev, E. Dobreva, G. Asseva, I. Ivanov, P. Petrov, A. Mangarov, I. Tomova, T. Kantardjiev | ||
Abstract: OBJECTIVE: To develop and optimize a rapid molecular method for diagnosing campylobacteriosis directly from a clinical fecal sample and at the same time for determining the most common causing agents – C. jejuni/coli. MATERIALS AND METHODS: 38 clinical fecal samples from hospitalized patients with diarrheal syndrome were tested using a rapid immunochromatographic test. All positive samples were tested for confirmation by culturing in a microaerophilic atmosphere. The Eva Green real-time mPCR reaction of a direct fecal sample was conducted using the “IQ5TM Real-Time PCR System” apparatus. RESULTS: Out of 38 clinical fecal samples which were ICT positive, 18 strains were isolated by culture, namely, 17 of C. jejuni and 1 of C. coli. The Eva Green real-time mPCR reaction also reported 18 positive samples for Campylobacter, out of which 17 were of C. jejuni and only one of C.coli. CONCLUSION: We developed and optimized the Eva Green real-time mPCR for the detection and species differentiation of C. jejuni/coli directly from a clinical fecal sample. The molecular analysis we described has a 100% sensitivity and specificity when comparing the results obtained by it to those of the culture method, which is currently the “gold standard” in the diagnosis of campylobacteriosis (Tab. 2, Fig. 1, Ref. 6). |
||
Keywords: Campylobacter, diarrhea, mPCR | ||
Published online: 05-Dec-2017 | ||
Year: 2017, Volume: 118, Issue: 11 | Page From: 702, Page To: 704 | |
doi:10.4149/BLL_2017_132 |
||
|
![]() |
|