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FOR AUTHORS General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.1, p.57–69, 2018
FOR AUTHORS General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.1, p.57–69, 2018
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General Physiology and Biophysics Vol.37, No.1, p.57–69, 2018 |
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| Title: DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency | ||
| Author: Lukáš Hubčík, Dominika Galliková, Petra Pullmannová, Ľubica Lacinová, Zdena Sulová, Mária Hanulová, Sergio S. Funari, Ferdinand Devínsky, Daniela Uhríková | ||
| Abstract: DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45–60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability. |
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| Keywords: Gemini surfactants — Lipoplexes — Small angle X-ray diffraction — Fluorescence spectroscopy — Transfection | ||
| Published online: 22-Jan-2018 | ||
| Year: 2018, Volume: 37, Issue: 1 | Page From: 57, Page To: 69 | |
| doi:10.4149/gpb_2017042 |
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