Home Bratislava Medical Journal 2018 Bratislava Medical Journal Vol.119, No.3, p.143-151, 2018

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

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Bratislava Medical Journal Vol.119, No.3, p.143-151, 2018

Title: Neuronal regeneration in injured rat spinal cord after human dental pulp derived neural crest stem cell transplantation
Author: S. Kabatas, C. S. Demir, E. Civelek, I. Yilmaz, A. Kircelli, C. Yilmaz, Y. Akyuva, E. Karaoz

Abstract: OBJECTIVE: This study aimed to analyze the effect of human Dental Pulp-Neural Crest Stem Cells (hDP-NCSCs) delivery on lesion site after spinal cord injury (SCI), and to observe the functional recovery after transplantation.
METHODS: Neural Crest Stem Cells (NCSCs) were isolated from human Dental Pulp (hDP). The experimental rat population was divided into four groups (n = 6/24). Their behavioral motility was scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords were examined for Green Fluorescent Protein (GFP) labeled hDP-NCSCs by immunofluorescence (IF) staining.
RESULTS: In early post-injury (p.i) period, the ultrastructure of spinal cord tissue was preserved in Group 4. The majority of cells forming the ependymal region around the central canal were found to be hDP-NCSCs. While the grey-and-white-matter around the ependymal region was composed of e.g. GFP cells, with astrocytic-like appearance. The scores showed significant motor recovery in hind limb functions in Group 4. However, no obvious change was observed in other groups.
CONCLUSION: Cells e.g., mesenchymal (Vimentin+) which express GFP+ cells in the gray-and-white-matter around the ependymal region could indicate the potential to self-renewal and plasticity. Thus, transplantation of hDP-NCSCs might be an effective strategy to improve functional recovery following spinal cord trauma (Fig. 10, Ref. 32).

Keywords: functional recovery, human dental pulp, neural crest stem cells, spinal cord injury
Published online: 06-Mar-2018
Year: 2018, Volume: 119, Issue: 3 Page From: 143, Page To: 151

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