| Abstract: OBJECTIVE: To investigate the role of GLP-1 in rat with mesangial proliferative glomerulonephritis.
METHODS: A total of 27 normal male Sprague-Dawley rats were randomly divided into 3 groups: NC group (normal control group); MC group (nephritis model rats) and GLP-1 group (nephritis rats treated with GLP-1) with 9 rats in each group. 24-hour urinary protein excretion, serum cystatin C and serum creatinine were tested at the end of 12 weeks. The proliferation of mesangial cells was measured by H&E and TUNEL staining of paraffin sections. The expressions of IKK-β, NF-κB, IL-6 and TNF-α were detected by immunohistochemisty and RT-PCR.
RESULTS: Compared with NC group, 24-hour urinary protein excretion, serum cystatin C and creatinine in MC groups significantly increased (p<0.05, respectively), the 24-hour urinary protein excretion, serum cystatin C and creatinine in GLP-1 group were significantly decreased compared with MC group (p<0.05, respectively). The relative protein and gene expressions of IKK-β, NF-κB, IL-6 and TNF-α of MC group were significantly up-regulated compared with NC group (p<0.05, respectively). However, compared with MC group, the IKK-β, NF-κB, IL-6 and TNF-α gene and proteins expression were significantly suppressed in GLP-1 group (p<0.05, respectively).
CONCLUSION: The mesangial glomerulonephritis is correlated with GLP-1 and GLP-1 up-regulation had effects to suppress mesangial glomerulonephritis by IKK-β pathway (Fig. 4, Ref. 22).
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