Home Acta Virologica 2018 Acta Virologica Vol.62, No.2, p.137-146, 2018

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.62, No.2, p.137-146, 2018

Title: Naked DNA immunization with full-length attachment gene of human respiratory syncytial virus induces safe and protective immune response
Author: M. E. HAMAD, H. M. AMER, M. A. FARRAG, A. H. OSMAN, F. N. ALMAJHDI

Abstract: Development of potent vaccine for human respiratory syncytial virus (HRSV) that confers better protection than natural infection remains a global challenge. Vaccination with naked DNA is considered successful approach for the control of many viral diseases. In this study, the potential of DNA vaccination using full-length attachment gene of HRSV type A Saudi strain cloned in pcDNA3.1+ vector (pcDNA/GA) was evaluated in BALB/c mice. The expression efficiency of pcDNA/GA was first confirmed in HEp-2 cells on RNA and protein levels. Mice immunization with either pcDNA/GA or the positive control formalin-inactivated vaccine (FI-RSV) has generated significant serum antibody concentration in ELISA (7.31±0.418 and 9.76±0.006 µg/ml, respectively) with superior neutralizing activity. Similarly, both immunogens evoked robust HRSV-specific CD8+ T-cell response in ELISPOT assay compared to mice immunized with pcDNA3.1+ vector or saline (negative controls). Challenge of the immunized mice with the wild-type HRSV did not provoke clinical symptoms or mortality in any mice group. On the 7th day post-challenge, mice were euthanized and lungs were extirpated for evaluation of viral load, histopathological changes and cytokine profile. A significant diminish in the viral load and histology score were concluded in lungs of pcDNA/GA immunized mice compared to those immunized with FI-RSV and negative controls. The pulmonary cytokine profile of pcDNA/GA immunized mice displayed notable upregulation of Th1-associated cytokines while that of FI-RSV immunized mice exhibited high levels of Th2-associated cytokines. In conclusion, the DNA vaccine candidate pcDNA/GA has proven prominent efficacy and safety in mouse model, which encourages further evaluation in clinical trials.

Keywords: attachment glycoprotein; challenge; DNA vaccination; human respiratory syncytial virus; mice immunization; T-helper biased response
Published online: 07-Jun-2018
Year: 2018, Volume: 62, Issue: 2 Page From: 137, Page To: 146
doi:10.4149/av_2018_203


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