Home General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.3, p.263–274, 2018

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Founded: 1982
ISSN  1338-4325 (online)

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General Physiology and Biophysics Vol.37, No.3, p.263–274, 2018

Title: Bone marrow mesenchymal stem cells modified pathological changes and immunological responses in ovalbumin-induced asthmatic rats possibly by the modulation of miRNA155 and miRNA133
Author: Mahdi Ahmadi, Reza Rahbarghazi, Amir-Ali Shahbazfar, Horeyeh Baghban, Rana Keyhanmanesh

Abstract: In the current experiment, we investigated the immune-modulatory potential of mesenchymal
stem cells (MSCs) and conditioned media (CM) in attenuating of chronic asthmatic changes
in a rat model. Male rats were divided into control (C) and ovalbumin-sensitized (S) groups, which
further allocated into three subgroups; rats received systemically 50 μl volume of PBS (C and S groups),
CM (CSV and SSV groups) and rats received intravenous infusion of 2 × 106 bone marrow-derived
mesenchymal stem cells (rBMMSCs) (CCV and SCV groups). Two weeks later, the expression of
interleukin (IL)-4, IL-13, and IL-10, miRNA133, and miRNA155 was measured by real-time PCR.
Pathological changes and the recruitment of rBMMSCs into pulmonary parenchyma were evaluated
by histopathological and immunofluorescence analyses, respectively. The systemic injection
of rBMMSCs, but not CM, decreased the levels of IL-4, IL-13, IL-10, miRNA133, miRNA155 and
reduced pathological changes in sensitized rats as compared with other sensitized groups (p < 0.001
to p < 0.05). rBMMSCs transmigrated to lung tissue in cell-administrated rats, albeit intensity of
asthmatic changes, in turn, affected the amount of recruited cells. Collectively, our data suggest the
potential role of MSCs, but not CM, in reducing pathological changes possibly via the modulation
of miRNA133 and miRNA155 during asthmatic changes.

Keywords: Asthma, Conditioned media, Mesenchymal stem cells, miRNA133, miRNA155
Published online: 31-May-2018
Year: 2018, Volume: 37, Issue: 3 Page From: 263, Page To: 274
doi:10.4149/gpb_2017052
Price: 24.00 €






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