Home General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.3, p.319–328, 2018

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Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.37, No.3, p.319–328, 2018

Title: The effect of ellagic acid on photodynamic therapy in leukemia cells
Author: Dan Sun, Yao Lu, Su-Juan Zhang, Kai-Ge Wang, Yuan Li

Abstract: Ellagic acid (EA) is a naturally phenolic acid presented in different foods. It has a variety of
biological activities including antioxidant, anti-inflammatory, anti-microbiological and anti-cancer
properties. On account of its antioxidant activity, EA might protect cancer cells from free radical
damage in photodynamic therapy (PDT) during which reactive oxygen species (ROS) production
was stimulated leading to irreversible tumor cell injury. In this study, the influence of EA on K562
cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. Cell apoptosis was assayed by flow
cytometry. Oxidative damage induced by PDT was investigated by measurement of malondialdehyde
(MDA). Comet assay was used to evaluate the potential genotoxic effect induced by PDT on the
cells. The results showed that EA supplementation alone did not affect the lipid peroxidation, DNA
damage and apoptosis in K562 cells. It increases the lipid peroxidation, DNA damage, apoptosis and
decreases the survival rate in K562 cells induced by ALA-PDT. The singlet oxygen quencher sodium
azide suppresses apoptosis, lipid peroxidation and DNA damage induced by EA in PDT. In conclusion,
EA consumption during PDT did not decrease the effectiveness of cancer therapy on malignant cells.
The effect of antioxidants on PDT maybe was determined by its sensitization ability to singlet oxygen.

Keywords: Ellagic acid, Reactive oxygen species, Photodynamic therapy, Sodium azide, Leukemia K562
Published online: 31-May-2018
Year: 2018, Volume: 37, Issue: 3 Page From: 319, Page To: 328

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