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CONTACT Bratislava Medical Journal 2018 Bratislava Medical Journal Vol.119, No.6, p.355-360, 2018
CONTACT Bratislava Medical Journal 2018 Bratislava Medical Journal Vol.119, No.6, p.355-360, 2018
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Bratislava Medical Journal Vol.119, No.6, p.355-360, 2018 |
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| Title: miRNA-31 over-expression improve synovial cells apoptosis induced by RA | ||
| Author: C. Luo, J. S. Liang, J. Gong, H. L. Zhang, Z. J. Feng, H. T. Yang, H. B. Zhang, Q. H. Kong | ||
| Abstract: OBJECTIVE: The aim of this study was to evaluate the effects and mechanism of miRNA-31 in synovial cells apoptosis induced by RA. METHODS: The miRNA-31 gene expressions were extracted from synovial tissues of normal and RA patients by RT-PCR and H et E staining. The synovial cells of RA patients were isolated and randomly divided into Control, Blank and miRNA groups. The cell apoptosis of difference groups were measured by flow cytometry; the TNF-α and IL-1β concentrations of difference groups were measured by Elisa assay; TLR4 and NF-κB proteins expressions were measured by WB assay and the correlation between TLR4 and miRNA-31 were evaluated by double luciferase target experiment. RESULTS: The miRNA-31 gene expression was significantly suppressed in RA tissues (p<0.001); Compared with control group, the cell apoptosis rate of miRNA group was significantly suppressed (p<0.001); TNF-α and IL-1β concentrations were significantly down-regulation in culture fluid (p<0.001, respectively) and TLR4 and NF-κB proteins expressions were significantly depressed (p<0.001, respectively) in miRNA group. By double luciferase target experiment, the TLR4 was a target gene of miRNA-31. CONCLUSION: miRNA-31 is a key role in synovial cells apoptosis induced by RA (Fig. 7, Ref. 23). |
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| Keywords: miRNA-31, cell apoptosis, RA; TLR4, NF-κB | ||
| Published online: 27-Jun-2018 | ||
| Year: 2018, Volume: 119, Issue: 6 | Page From: 355, Page To: 360 | |
| doi:10.4149/BLL_2018_066 |
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