Home CUSTOMERS Bratislava Medical Journal 2018 Bratislava Medical Journal Vol.119, No.9, p.550–553, 2018

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.119, No.9, p.550–553, 2018

Title: Neural derivation of human dental pulp stem cells via neurosphere technique
Author: M. N. Bojnordi, S. Haratizadeh, S. Darabi, H. G. Hamidabadi

Abstract: INTRODUCTION: Human dental pulp stem cells (hDPSCs) are multipotent stem cells providing an autologous noninvasive cell source. The study evaluates the neurogenic potential of hDPSCs using neural growth factor inducers and neurosphere technique.
METHODS: The hDPSCs were differentiated into neurons using neural induction medium containing retinoic acid (RA). Neuroprogenitor cells were evaluated for nestin and NF68 using immunocytochemistry. The mature neuron markers, MAP‑2 and β-tubulin, were investigated at the end stage of induction phase.
RESULTS:The neuroprogenitor differentiation was confirmed by immunostaining for nestin and NF68 markers. The differentiated neurons were positive for specific neuron markers, namely for MAP‑2 and β-tubulin. The results indicated that the neural differentiation medium and neurosphere technique improve the generation of neuroprogenitor cells as well as mature neurons via exhibiting specific neural markers, namely nestin, NF68, MAP‑2 and β-tubulin.
CONCLUSION: Our findings highlight the differentiation capacity of hDPSCs via neurosphere technique in the presence of neural inducers for mesenchymal stem cells. It is suggested that the neural differentiation potential of hDPSCs can be exploited as a source of stem cells for therapy of neurodegenerative diseases (Fig. 5, Ref. 20).

Keywords: mesenchymal stem cells, dental pulp stem cells, neuron, neuroprogenitor cells, multipotent stem cells
Published online: 13-Sep-2018
Year: 2018, Volume: 119, Issue: 9 Page From: 550, Page To: 553

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