Home General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.5, p.597–601, 2018

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.37, No.5, p.597–601, 2018

Title: Thermodynamic properties of DNA-dendrimer complexes and features of their applications
Author: T. Mdzinarashvili, M. Khvedelidze, E. Shekiladze, N. Shengelia, T. Hianik


The commercial solution of fourth generation (G4) of poly-amidoamine (PAMAM) dendrimers contains methanol, which is toxic for human body. Our differential scanning calorimetry (DSC) study of dendrimers confirmed the existence of this threat. The recommendation is done on how to prepare dendrimer solutions for practical and safely use in gene delivery. DSC have been also used to study the thermodynamic properties of DNA/dendrimer complexes (dendriplexes). We showed that up to DNA/dendrimer ratio 43 ± 3 (w/w) the solution was homogeneous, but stable aggregates were formed at higher PAMAM content.

DSC experiments performed with homogeneous solution of dendriplexes revealed existence of the pH-dependent melting curves that contain several endothermic peaks associated with melting of GC-rich regions.

Keywords: PAMAM dendrimers, Methanol, DNA, Gene delivery, Differential scanning calorimetry
Published online: 02-Oct-2018
Year: 2018, Volume: 37, Issue: 5 Page From: 597, Page To: 601

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