Home Neoplasma 2018 Neoplasma Vol.65, No.6, p.943-951, 2018

Journal info

6 times a year.
Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

Editorial Info
Abstracted and Indexed
Submission Guidelines

Select Journal

Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Neoplasma Vol.65, No.6, p.943-951, 2018

Title: Comparison of HLA-G and MMP transcription in human tumor cell lines
Author: K. Polakova, E. Bandzuchova

Abstract: HLA-G antigens and matrix metalloproteinases (MMPs) expressed in various tumors are involved in tumor growth and metastasis. In this study, we investigated if correlation between HLA-G and MMP expression exists in different cell lines. We examined MMP transcription in two choriocarcinoma cell lines: JEG-3 (HLA-G positive) and JAR (HLA-G negative). We discovered that both cell lines express a similar panel of MMPs; except for MMP12. Transcript MMP12 was exclusively detected in HLA-G expressing JEG-3 cells but not in HLA-G deficient JAR cells. We observed HLA-G expression but no MMP12 transcription following 5-aza-2´-deoxycytidine (AZA) treatment of JAR cells. We then investigated HLA-G and MMP transcription in several human leukaemia cell lines. Leukaemia cells (lacking HLA-G expression) were converted to their HLA-G positive counterparts by AZA-treatment or by HLA-G transfection. It was found no correlation between HLA-G and MMP transcription in any examined leukaemia cell lines. The up-regulation of some MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) was observed following AZA-treatment.

Keywords: HLA-G, matrix metalloproteinase, DNA hypomethylation, RT-PCR
Published online: 08-Oct-2018
Year: 2018, Volume: 65, Issue: 6 Page From: 943, Page To: 951

download file

© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.