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neo_2018_180409N230

Title: Comparison of HLA-G and MMP transcription in human tumor cell lines
Author: K. Polakova, E. Bandzuchova

Abstract: HLA-G antigens and matrix metalloproteinases (MMPs) expressed in various tumors are involved in tumor growth and metastasis. In this study, we investigated if correlation between HLA-G and MMP expression exists in different cell lines. We examined MMP transcription in two choriocarcinoma cell lines: JEG-3 (HLA-G positive) and JAR (HLA-G negative). We discovered that both cell lines express a similar panel of MMPs; except for MMP12. Transcript MMP12 was exclusively detected in HLA-G expressing JEG-3 cells but not in HLA-G deficient JAR cells. We observed HLA-G expression but no MMP12 transcription following 5-aza-2´-deoxycytidine (AZA) treatment of JAR cells. We then investigated HLA-G and MMP transcription in several human leukaemia cell lines. Leukaemia cells (lacking HLA-G expression) were converted to their HLA-G positive counterparts by AZA-treatment or by HLA-G transfection. While we found no correlation between HLA-G and MMP transcription in any examined leukaemia cell lines, the up-regulation of some MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) was observed following AZA-treatment.

Keywords: HLA-G, matrix metalloproteinase, DNA hypomethylation, RT-PCR
Published online: 08-Oct-2018
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2018_180409N230


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