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Neoplasma Vol.66, No.1, p.28-32, 2019 |
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Title: Effect of RRS1 gene knockdown on BT549 cell line proliferation and apoptosis in breast cancer | ||
Author: Y. N. Hua, J. L. Song, Z. L. Ma, L. Wu, Z. Zhang, L. Zhang, N. Li, S. B. Cong, L. Hou | ||
Abstract: The RRS1 regulator of ribosome synthesis has recently been reported a new target gene linked to cancer development. This study therefore investigates RRS1effectsb on BT549 cell proliferation and apoptosis in breast cancer. Western blot (WB) and real - time quantitative PCR (qPCR) were used to detect the relative expression of RRS1 in breast cancer cells BT-549 and the normal HMEC mammary gland epithelial cells. BT-549 cells were cultured and infected with retroviruses and RRS1 expression was detected by qPCR and WB. The MTT assay, Caspase-3/7 and flow cytometry (FCM) then detected growth and apoptosis in the BT549 breast cancer BT cell. WB detected the expression of Bcl-2 and Bax genes related to apoptosis at the protein level, and MTT assay confirmed that RRS1 knockdown significantly decreased cell viability (p |
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Keywords: RRS1, BT549, apoptosis, bcl-2 | ||
Published online: 22-Jan-2019 | ||
Year: 2019, Volume: 66, Issue: 1 | Page From: 28, Page To: 32 | |
doi:10.4149/neo_2018_171229N853 |
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