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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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neo_2018_171229N853

Title: Effect of RRS1 gene knockdown on BT549 cell line proliferation and apoptosis in breast cancer
Author: J. L. SONG, Y. N. HUA, Z. L. MA, L. WU, Z. ZHANG, L. ZHANG, S. B. CONG, L. HOU

Abstract: The RRS1 regulator of ribosome synthesis has recently been reported a new target gene linked to cancer development. This study therefore investigates RRS1effectsb on BT549 cell proliferation and apoptosis in breast cancer. Western blot (WB) and real - time quantitative PCR (qPCR) were used to detect the relative expression of RRS1 in breast cancer cells BT-549 and the normal HMEC mammary gland epithelial cells. BT-549 cells were cultured and infected with retroviruses and RRS1 expression was detected by qPCR and WB. The MTT assay, Caspase-3/7 and flow cytometry (FCM) then detected growth and apoptosis in the BT549 breast cancer BT cell. WB detected the expression of Bcl-2 and Bax genes related to apoptosis at the protein level, and MTT assay confirmed that RRS1 knockdown significantly decreased cell viability (P

Keywords: RRS1; BT549; apoptosis; bcl-2
Published online: 17-Oct-2018
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2018_171229N853


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