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neo_2018_180302N146

Title: Tumor suppressor let-7a inhibits breast cancer cell proliferation, migration and invasion by targeting MAGE-A1
Author: Y. Z. MI, F. LIU, X. L. LIANG, S. N. LIU, X. C. HUANG, M. X. SANG, C. Z. GENG

Abstract: Let-7 was one of the earliest discovered miRNAs and while it reportedly acts as a tumor suppressor in various solid tumors, its function in breast cancer has not been fully studied. Therefore, we examined let-7a and MAGE-A1 expression in breast tissues by qRT-PCR and found that let-7a expression significantly correlates with larger tumor size, higher histological grade (P 14% (P = 0.028 and P = 0.023). MAGE-A1 expression incidence is 50.8% (33/65) and it inversely correlates with let-7a expression (P = 0.008). let-7a inhibition of breast cancer cell proliferation, migration and invasion was also observed in in vitro cell culture experiments, and dual-luciferase reporter assays showed that melanoma-associated antigen A1 (MAGE-A1) was its target gene; the target comprised bases 451-457 of the 3’UTR region of the MAGE-A1 mRNA. RT-qPCR and Western blot analyses showed that let-7a inhibited MAGE-A1 expression at both the nucleic acid and protein levels. In our final co-transfection experiment, we targeted MAGE-A1 in a breast cancer cell line and observed that let-7a inhibited cell proliferation, migration and invasion. These combined results confirm that let-7a functions as a tumor suppressor by targeting MAGE-A1 in breast cancer and it therefore provides a novel target in breast cancer clinical treatment.

Keywords: breast cancer, miRNA, let-7a, MAGE-A1
Published online: 17-Oct-2018
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2018_180302N146


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