Home Bratislava Medical Journal 2018 Bratislava Medical Journal Vol.119, No.10, p.625–629, 2018

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.119, No.10, p.625–629, 2018

Title: The impact of apelin-36 on isolated rat hearts as a member of apelin family
Author: O. Kutlay

Abstract: BACKGROUND: Apelin is an endogenous adipocytokine that plays an important role in the regulation of cardiovascular function. Apelin-36 is a member of apelin family. However cardiac reports related to apelin-36 are very rare. The purpose of this study is to investigate the impact of apelin-36 on hemodynamic variables of the isolated rat hearts.
METHODS: Twenty-eight rats were equally divided into four groups: control, apelin-36 at the following concentrations: 1 nM, 10 nM and 100 nM. The isolated hearts were perfused with modified Krebs-Henseleit solution (mK-Hs) by using the Langendorff method. Cardiac parameters, including left ventricular developed pressure (LVDP), maximal rate of pressure development (+dP/dtmax), heart rate (HR) and coronary flow (CF) were measured. Gene expressions relevant to cardiomyocyte contractility were determined by real-time PCR.
RESULTS: 10 and 100 nM doses of apelin-36 perfusion led to positive inotropy with an increase of LVDP and +dP/dtmax, which are the indexes of cardiac contractility. Furthermore both doses of apelin-36 increased endothelial nitric oxide synthase (eNOS), sarco/endoplasmic reticulum Ca2+-ATPase (Serca2a) and β2-Adrenergic receptors (β2-AR) mRNA.
CONCLUSION: These results showed that apelin-36 had a positive inotropic effect on the isolated rat heart and can induce eNOS, Serca2a and β2-AR genes activation that enhances contractility of the heart (Tab. 1, Fig. 2, Ref. 23).

Keywords: apelin-36, Serca2a, β2-AR, cardiomyocyte, contractility
Published online: 18-Oct-2018
Year: 2018, Volume: 119, Issue: 10 Page From: 625, Page To: 629

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