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Endocrine Regulations Vol.42, p.45-51, 2008 |
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Title: NITRIC OXIDE STIMULATES THE PRODUCTION OF ATRIAL NATRIURETIC PEPTIDE AND PROGESTERON BY HUMAN GRANULOSA LUTEINIZED CELLS WITH AN ANTIAPOPTOTIC EFFECT | ||
Author: J. D. DINEVA, I. M. VANGELOV, G. G. NIKOLOV, R. T. KONAKCHIEVA, M. D. IVANOVA | ||
Abstract: Objective. To study the effect of nitric oxide (NO) on atrial natriuretic peptide (ANP) and progesterone (P) production by human granulose luteinized cells (GLC) in vitro and to elucidate their role on the survival of cultured cells. Methods. Human GLCs were cultured in HAM’s F10/10 % FCS as monolayers for 24 h. Subsequently GLCs were treated for 24 h with 0.5 mM Sodium Nitroprusside (SNP, NO donor) and 0.5 mM Aminoglutethimide (AG , P450scc inhibitor). The levels of ANP and P were measured in supernatants of cultured cells by proANP(1-98) kit and RIA, respectively. Caspase-3 activity was determined by Ac-DEVD-pNA as substrate. Results. The production of ANP and P was increased by NO as compared to control cells (p |
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Keywords: ANP – Apoptosis – Granulosa luteinized cells – Nitric oxide – Progesterone | ||
Year: 2008, Volume: 42, Issue: 2 | Page From: 45, Page To: 51 | |
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