Home General Physiology and Biophysics 2018 General Physiology and Biophysics Vol.37, No.6, p.695–702, 2018

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.37, No.6, p.695–702, 2018

Title: Analysis of the adhesion activity of peritoneal macrophages after exposure to radiation from a gas-discharge plasma and mercury lamp
Author: E. Arkhipova, I. Piskarev, I. Ivanova

Abstract: The aim of the work was to study the influence of UV radiation of a spark discharge plasma and a mercury lamp on the state of membrane structures of peritoneal macrophages. The objects of the study were peritoneal macrophages of rats. The total number of cells after exposure and their viability were analyzed. Oxidative modification of proteins was recorded by fluorescence of tryptophan, tyrosine and products of non-enzymatic glycosylation of proteins. The concentration of sialic acids was determined spectrophotometrically, and the intensity of adhesion properties of cells was estimated by the ability to adhere to the plastic. It was shown that the radiation of a spark discharge plasma and UV lamp with the selected exposure regimes affect the structural components of membranes of peritoneal macrophages. The ability to adhere is enhanced by short exposure regimes, and under long-term conditions, adhesion properties decrease. The change in adhesion is probably associated with a decrease in the concentration of sialic acids on the cell surface, as well as with the intensification of oxidative modification of proteins. It has been established that spark plasma and UV lamp radiation promote the oxidation of aromatic amino acids and the accumulation of glycosylation products of proteins.

Keywords: Peritoneal macrophages, Gas-discharge plasma, UV radiation, Fluorescence of amino acids, Adhesion
Published online: 28-Nov-2018
Year: 2018, Volume: 37, Issue: 6 Page From: 695, Page To: 702

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