Home Acta Virologica 2019 Acta Virologica Vol.63, No.1, p.36-44, 2019

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.63, No.1, p.36-44, 2019

Title: Use of the cotton leaf curl Multan alphasatellite as a silencing or expression vector

Abstract: Alphasatellites, formerly known as DNA 1, are a satellite-like components associated with begomoviruses (the family Geminiviridae) that require betasatellite for symptom induction but depend on DNA-A for systemic movement. We have converted alphasatellite into gene-silencing vector (modified alphasatellite (∆DNA 1)) by deleting its A-rich region that does not affect the replication nor the movement of the helper virus. Insertion of a transgene green florescence protein (GFP) into ∆DNA 1 resulted in the silencing g of the cognate gene in Nicotiana benthamiana. The silencing persisted for more than one and half month and was associated with the decreased level of mRNA of the target gene. This satellite-like DNA vector induced gene silencing (VIGS) promises to be applicable to other begomovirus/alphasatellite systems, thereby providing the powerful approach to gene discovery and the analysis of gene functions in malvaceous crops.

Keywords: cotton; begomovirus; alphasatellite; RNAi
Published online: 13-Mar-2019
Year: 2019, Volume: 63, Issue: 1 Page From: 36, Page To: 44

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