Home Acta Virologica 2019 Acta Virologica Vol.63, No.1, p.80-87, 2019

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.63, No.1, p.80-87, 2019

Title: A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.
Author: N.-T. YU, Y.-L. ZHANG, Z. XIONG, L. QU, Z.-X. LIU

Abstract: Grouper nervous necrosis virus (GNNV) and grouper iridovirus (GIV) are major grouper-infecting viruses in southern China that can cause serious economic losses. A duplex reverse transcription-PCR (duplex RT-PCR) method was developed for the simultaneous detection of GNNV and GIV. Eight groups of primers specifically targeting the capsid protein genes of GNNV and GIV were designed and analyzed. The primer set GN4 was selected and used to amplify fragments of 887 bp and 319 bp in length from GNNV and GIV, respectively. Furthermore, the duplex PCR assay was shown to be sensitive because it could detect at least 20 pg of plasmid-viral DNA from a mixture of viruses. Using this assay, 18 GNNV infected groupers and 7 GIV infected groupers were detected amongst 41 suspected samples in Hainan. The duplex RT-PCR assay proved to be a rapid, specific, and sensitive method for detecting the two grouper viruses. This method could be used to facilitate better control of fish viruses through early detection.

Keywords: duplex reverse transcription-PCR; nervous necrosis virus; iridovirus; grouper
Published online: 13-Mar-2019
Year: 2019, Volume: 63, Issue: 1 Page From: 80, Page To: 87

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