Home HOME Acta Virologica 2019 Acta Virologica Vol.63, No.1, p.117-120, 2019

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.63, No.1, p.117-120, 2019

Title: Phospholipase C signaling is involved in porcine reproductive and respiratory syndrome virus infection in cell cultures
Author: W. F. YUAN, Q. CHEN, X. T. GAO, Z. M. ZHENG, H. JIA, H. F. ZHU, T. XIN, X. K. SUI, M. LI, S. H. HOU, X. Y. GUO

Abstract: The phospholipase C (PLC) is a family of kinases that hydrolyze phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to generate two second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), which stimulate distinct downstream signaling. Recently, it has been reported that PLC signaling is activated by multiple viruses for efficient replication and the virus-induced inflammatory response. In this study, we demonstrated that PLC-specific inhibitor U73122 strongly suppressed porcine reproductive and respiratory syndrome virus (PRRSV) productive infection in cell cultures. The inhibitor affected both viral post-binding cell entry and post-entry processes. The virus infection led to an early transient activation of PLCγ-1 at 0.5 h post-infection (hpi), and sustained event at a stage from 4 to 16 hpi in MARC-145 cells. In addition, U73122 inhibited the activation of p38 MAPK signaling stimulated by PRRSV infection, suggesting that PLC signaling may be associated with the virus infection-induced inflammatory response. Taken together, these studies suggested that PLC signaling played an important role in PRRSV infection or pathogenesis.

Keywords: PRRSV; U73122; phospholipase C; PLCγ-1
Published online: 13-Mar-2019
Year: 2019, Volume: 63, Issue: 1 Page From: 117, Page To: 120
doi:10.4149/av_2019_115


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