Home HOME Neoplasma 2019 Neoplasma Vol.66, No.3, p.350-356, 2019

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.66, No.3, p.350-356, 2019

Title: miR-375 inhibits the proliferation and invasion of glioblastoma by regulating Wnt5a
Author: G. F. Li, Y. Y. Cheng, B. J. Li, C. Zhang, X. X. Zhang, J. Su, C. Wang, L. Chang, D. Z. Zhang, C. L. Tan, N. Wang

Abstract: The aberrant expression of microRNA-375 (miR-375) has been proven to be associated with carcinogenesis. However, the role of miR-375 in glioblastoma (GBM) remains unknown. The aim of this study was to investigate miR-375 biological functions and its molecular mechanisms in GBM cells. In this study, real-time PCR results showed that the level of miR-375 expression in GBM tissues and GBM cell lines (U87 and U251) was decreased. Using MTT assay, trans-well migration and invasion assay, we demonstrated that miR-375 overexpression significantly suppress cell proliferation, cell migration and cell invasion capacity in U87 and U251 cells. However, downregulation of miR-375 had reverse effects on cell proliferation, migration and invasion.  In association analysis, dual luciferase assay, RT-PCR and western blot analysis results confirmed that miR-375 could target the 3’UTR of Wnt5a mRNA and regulate its protein expression. Further studies also showed that Wnt5a overexpression significantly reversed miR-375-mediated proliferation, migration and invasion in U87 and U251 cells. Therefore, we concluded that miR-375 inhibits proliferation and invasion of GBM by regulating Wnt5a and therefore might be a possible therapeutic agent for GBM.

Keywords: miR-375, Wnt5a, proliferation, invasion, glioblastoma
Published online: 28-May-2019
Year: 2019, Volume: 66, Issue: 3 Page From: 350, Page To: 356

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