Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.66, No.3, p.437-445, 2019 |
||
Title: Targeting genes and signaling pathways of transcriptional suppressor ZHX2 in hepatocellular carcinoma: a Chromatin Immunoprecipitation-sequencing (ChIP-seq) investigation | ||
Author: Z. Lv, R. He, M. Huang, G. Zhao, J. Ma, G. Chen | ||
Abstract: Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. The unclear underlying molecular mechanisms could provide important theoretical basis for prevention and control of HCC. In this study, chromatin immunoprecipitation-sequencing (ChIP-seq) was performed to analyze the binding sites of zinc fingers and homeoboxes 2 (ZHX2) and its genome-wide target genes, and bioinformatics was used to analyze their gene transcription regulation network. Immunohistochemistry detected the ZHX2 expression in HCC, and its association with clinicopathological characteristics of HCC. Results of RT-PCR and western blot showed that the ZHX2 expression in HepG2 cells was obviously lower compared to normal liver cells. ZHX2 could be amplified in ChIP products and ChIP-seq revealed there were 232 genes binding in promoter regions. GO function analysis revealed these genes were mainly associated with biological processes (BP), cellular components (CC), and molecular functions (MF). In addition, PTEN was found enriched in certain biological functions in BP analysis. Then, four pathways of these genes based on Kyoto Encyclopedia of Genes and Genomes (KEGG) were found p |
||
Keywords: hepatocellular carcinoma, HCC, transcription repressor, ZHX2, ChIP-Seq | ||
Published online: 28-May-2019 | ||
Year: 2019, Volume: 66, Issue: 3 | Page From: 437, Page To: 445 | |
doi:10.4149/neo_2018_180806N593 |
||
|
download file |
|