Home FOR AUTHORS General Physiology and Biophysics 2019 General Physiology and Biophysics Vol.38, No.2, p.145–155, 2019

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Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

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General Physiology and Biophysics Vol.38, No.2, p.145–155, 2019

Title: Uranium exposure increases spermatocytes metaphase apoptosis in rats: inhibitory effect of thymoquinone and N-acetylcysteine
Author: H. Waly, S. M. M. Ragab, K. M. A. Hassanein, N. S. Abou Khalil, E. A. Ahmed

Abstract: Uranyl acetate (UA), a commercial stock from depleted uranium (DU), has a combined effect of chemical toxicity and mild radioactivity. Here, we investigated the potential antioxidant, antiapoptotic and cytoprotective effects of thymoquinone (TQ) and N-acetylcysteine (NAC) against UA-induced testicular damage in rats. UA reduced testicular superoxide dismutase (SOD) activity and nitric oxide (NO) and glutathione (GSH) levels relative to the control group. Interestingly, the testicular SOD activity and NO and GSH levels of UA/TQ- and UA/NAC-treated groups were also significantly lower relative to the control. A marked increase in spermatocytes metaphase apoptosis was found (stage XIII) in UA-treated rats, which is probably due to difficulties in segregation of homologous-chromosomes. This may clarify why UA exposure decreased round spermatids numbers and fertility in previous studies. To check the reason of partial metaphase arrest, the presence of DNA-damage-related γ-H2AX foci in late spermatocytes of all groups was checked, but only insignificant increase was found in UA-treated group. TQ or NAC supplementation reduced the apoptosis and improved the testicular histological alterations. Thus, TQ and NAC attenuate UA adverse effects on the testicular microenvironment through anti-apoptotic and cytoprotective but not antioxidant effects.

Keywords: Uranyl acetate, Testis, Metaphase apoptosis, Thymoquinone, N- Acetylcysteine
Published online: 25-Mar-2019
Year: 2019, Volume: 38, Issue: 2 Page From: 145, Page To: 155

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