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FOR AUTHORS Acta Virologica 2019 Acta Virologica Vol.63, No.3, p.261-269, 2019
FOR AUTHORS Acta Virologica 2019 Acta Virologica Vol.63, No.3, p.261-269, 2019
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Acta Virologica Vol.63, No.3, p.261-269, 2019 |
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| Title: The effect of human interferon alpha on replication of different bovine viral diarrhea virus strains | ||
| Author: A. A. ELSHEIKH, L. J. BRAUN, S. M.G. MANSOUR, A. ORABI, A. S. ALQAHTANI, D. A. BENFIELD, C. C.L. CHASE | ||
| Abstract: Bovine viral diarrhea virus (BVDV) exists in two main biotypes: cytopathic (cp) and noncytopathic (ncp). Although some studies were done on the effect of interferon alpha (IFN-α) on BVDV, the effect of exogenous IFN against BVDV biotypes remains unclear. In the present study, we evaluated the comparative effect of exogenous human IFN-α (HuIFN-α) on different BVDV biotypes and genotypes. The results showed that exogenous HuIFN-α greatly inhibited the growth of different BVDV biotypes and genotypes. However, HuINF-α has a significant inhibitory effect on cp biotype compared to ncp one without significant variation between different genotypes. The effect of HuIFN-α on BVDV reached the maximum level at early stages of infection (0–20 h post infection) and increased in a dose-dependent manner (10–500 U/ml). Quantitative real-time RT-PCR was used to evaluate the effect of exogenous HuIFN-α on RNA synthesis of both BVDV biotypes. HuIFN-α reduced RNA production of cp by 4 logs compared to only 2 logs for ncp strains. Additionally, the antiviral effect of IFN-α against both BVDV biotypes seems to be independent of the RNA-dependent protein kinase (PKR) activation as assayed by direct analysis of in vivo phosphorylation of eIF2-α and by 2-aminopurine (2-AP) treatment. Collectively, these results indicated that the exogenous HuIFN-α treatment has an inhibitory effect not only on cp BVDV biotype but also on the ncp BVDV. The antiviral effect of exogenous HuIFN-α was biotype, time, dose but not genotype dependent. PKR has no role in the inhibitory effect suggesting that other IFN-antiviral pathways were involved. |
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| Keywords: BVDV biotypes; HuIFN-α; RNA synthesis; PKR-independent | ||
| Published online: 09-Sep-2019 | ||
| Year: 2019, Volume: 63, Issue: 3 | Page From: 261, Page To: 269 | |
| doi:10.4149/av_2019_303 |
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