Home Bratislava Medical Journal 2019 Bratislava Medical Journal Vol.120, No.9, p.686-689, 2019

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345
 

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Bratislava Medical Journal Vol.120, No.9, p.686-689, 2019

Title: Adipose derived mesenchymal stem cells harvesting
Author: D. Palencar, J. Dragunova, I. Hulin, J. Koller

Abstract: BACKGROUND: The lipografting is increasingly used in the field of plastic surgery. Widely used harvesting technique of fatderived stem-cells is lipoaspiration. There exist two big streams of fat harvesting for lipografting: mechanical liposuction and manual liposuction.
METHODS: Two harvested specimens were compared in this prospective blind study in the means of stem-cells viability and their ability to grow in cell-cultures. Techniques to compare were: manual lipoaspiration with 50 ml syringe and WAL (water-jet assisted liposuction).
RESULTS: Twenty specimens from ten patients were investigated in the tissue bank. There were no differences in the amount of live stem-cells between two groups. Also no differences were found between both harvesting techniques in the mean of cell ability to grow in cell-cultures.
CONCLUSION: It can be concluded that there are no statistically significant differences in the number, vitality and viability of stem cells when comparing two ways of mesenchymal stem cell collection, both manual and machine sampling (WAL). When cultured in vitro, both samples collected from each patient also appeared to be able to multiply with no statistical differences (Tab. 2, Fig. 2, Ref. 18).

Keywords: lipograft, mesenchymal stem cells, WAL, manual lipoaspiration, regenerative medicine
Published online: 28-Aug-2019
Year: 2019, Volume: 120, Issue: 9 Page From: 686, Page To: 689
doi:10.4149/BLL_2019_115


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