Home FOR AUTHORS Acta Virologica 2019 Acta Virologica Vol.63, No.4, p.475-479, 2019

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Aims and Scope
Editorial Info

Abstracted and Indexed
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.63, No.4, p.475-479, 2019

Title: Efficient inhibition of foot-and-mouth disease virus replication in vitro by artificial microRNA targeting 3D polymerase
Author: S. H. BASAGOUDANAVAR, H. B. RANJITHA, M. HOSAMANI, SUJIT M. KOLANGATH, R.P. TAMIL SELVAN, B.P. SREENIVASA, P. SARAVANAN, A. SANYAL, R. VENKATARAMANAN

Abstract: Foot-and-mouth disease (FMD) is a devastating acute viral disease of livestock with cloven hooves. Among various therapeutic control measures, RNA interference (RNAi) is one of the methods being explored to inhibit FMD virus replication and spread. The RNAi is achieved by short hairpin RNAs or artificial microRNAs (amiRNAs). Utility of amiRNAs as antiviral, targeting conserved regions of the viral genome is gaining importance. However, delivery of miRNA in vivo is still a challenge. In this study, the efficacy of amiRNAs in preventing FMD virus replication in a permissive cell culture system was investigated, by generating stable cell lines expressing amiRNAs targeting three functional regions of the FMD virus (FMDV) genome (IRES, 3B3 and 3D). The results showed that amiRNA targeting 3D polymerase is relatively more efficient. However, expression of multiple microRNAs targeting the three regions did not exhibit additive effect. The data suggest that 3D specific miRNA is a potential valid strategy in developing novel antiviral measures against FMDV infection.

Keywords: artificial microRNA; foot-and-mouth disease virus; virus inhibition
Published online: 09-Oct-2019
Year: 2019, Volume: 63, Issue: 4 Page From: 475, Page To: 479
doi:10.4149/av_2019_407


download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.