Home Bratislava Medical Journal 2019 Bratislava Medical Journal Vol.120, No.10, p.752-756, 2019

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345
 

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Bratislava Medical Journal Vol.120, No.10, p.752-756, 2019

Title: Atorvastatin enhances anti-apoptotic effects of tamoxifen on melanoma cancer cells
Author: M. Ghasemi, M. Malek, Sh. Haghjooy Javanmard, A. Ghasemi, H. Naji Esfahani, G. Vaseghi

Abstract: AIM: Tamoxifen engages mitochondrial estrogen receptor beta as an antagonist, increases mitochondrial cytotoxicity and induces tumor cell death. Tamoxifen also engages plasma membrane estrogen receptor alpha as an agonist, while it is suggested that in some users its activation is put into action by mechanism of resistance to tamoxifen. Apoptotic inducers have been shown to promote tamoxifen-induced cell death, which might be of great importance in overcoming tamoxifen resistance. Considering the pleiotropic effects of statins, in the present study, we investigated the effects of atorvastatin on tamoxifen-induced intrinsic apoptotic pathway activity in melanoma cells.
METHODS: Melanoma B16F10 cells were treated for 24 and 48 h with various concentrations of tamoxifen, atorvastatin and combination of tamoxifen + atorvastatin. Cells with no treatment were considered a control group, and the study was then followed by quantitative RT- PCR assay. Bax and cytochrome c gene expressions were calculated by ΔΔct method.
RESULTS: Co-treatment of atorvastatin + tamoxifen could strongly enhance the expression of pro/apoptotic factors of Bax and cytochrome c in melanoma cells compared to the tamoxifen and atorvastatin groups.
CONCLUSION: In general, we conclude that the atorvastatin-induced increase in Bax and cytochrome c gene expression might be a permissive response to tamoxifen-induced cell death (Fig. 2, Ref. 37).

Keywords: tamoxifen, atorvastatin, melanoma, Bax, cytochrome c.
Published online: 28-Oct-2019
Year: 2019, Volume: 120, Issue: 10 Page From: 752, Page To: 756
doi:10.4149/BLL_2019_125


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